Read more about Tissue Engineering with a Flexcell® Culture System
Figure 1: Bioartificial tissue development with Collagel® and the Tissue Train® Culture System.
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Relevant Tech Reports & Other Information
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100: Tissue Train® Culture System. A Method for Culture and Mechanical Loading of Cells in a Linear 3D Matrix
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104: Trapezoidal Trough Loader?. A Device for Fabricating 3-Dimensional Bioartificial Tissue Constructs
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107: ScanFlex? with XyFlex?. An Automated Method to Measure Gel Compaction in Three Dimensional Bioartificial Tissues
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113: Tissue Train® Anchor Options. Comparison between the non-woven nylon and the urethane polyester foam anchors.
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114: Collagel® and ThermaCol®. Collagen I Hydrogel Kits for 3D Cell Culture.
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207: Methods for Immunochemical Staining of Cells in Three Dimensional Bioartificial Tissues (BATS)
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Collagel® Product Information Sheet
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Thermacol® Product Information Sheet
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Circular Foam Tissue Train® Culture Plates Product Information
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Linear Tissue Train® Culture Plates Product Information
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Trapezoidal Tissue Train® Culture Plates Product Information
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Flexcell® Transwell Holder Product Information
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Flexcell® Tissue Train® Culture System User Manual
Recent Publications with a Flexcell® Tissue Engineering Product
Quantitative assessment of forward and backward second harmonic three dimensional images of collagen type I matrix remodeling in a stimulated cellular environment
Abraham T, Kayra D, McManus B, Scott A. J Struct Biol 180(1):17-25, 2012. doi: 10.1016/j.jsb.2012.05.004.
Matrix rigidity activates Wnt signaling through down-regulation of Dickkopf-1 protein
Barbolina MV, Liu Y, Gurler H, Kim M, Kajdacsy-Balla AA, Rooper L, Shepard J, Weiss M, Shea LD, Penzes P, Ravosa MJ, Stack MS. J Biol Chem 288(1):141-51, 2013. doi: 10.1074/jbc.M112.431411.
Dosed myofascial release in three-dimensional bioengineered tendons: effects on human fibroblast hyperplasia, hypertrophy, and cytokine secretion
Cao TV, Hicks MR, Campbell D, Standley PR. J Manipulative Physiol Ther 36(8):513-21, 2013. doi: 10.1016/j.jmpt.2013.07.004.
Degree of scaffold degradation influences collagen (re)orientation in engineered tissues
de Jonge N, Foolen J, Brugmans MC, S?ntjens SH, Baaijens FP, Bouten CV. Tissue Eng Part A 20(11-12):1747-57, 2014. doi: 10.1089/ten.TEA.2013.0517.
Ablation of cardiac myosin-binding protein-C accelerates contractile kinetics in engineered cardiac tissue
de Lange WJ, Grimes AC, Hegge LF, Ralphe JC. J Gen Physiol 141(1):73-84, 2013. doi: 10.1085/jgp.201210837.
Engineering three-dimensional cell mechanical microenvironment with hydrogels
Huang G, Wang L, Wang S, Han Y, Wu J, Zhang Q, Xu F, Lu TJ. Biofabrication 4(4):042001, 2012. doi: 10.1088/1758-5082/4/4/042001.
Cyclical strain modulates metalloprotease and matrix gene expression in human tenocytes via activation of TGFβ
Jones ER, Jones GC, Legerlotz K, Riley GP. Biochim Biophys Acta 1833(12):2596-2607, 2013. doi: 10.1016/j.bbamcr.2013.06.019.
Cyclic mechanical strain induces TGFβ1-signalling in dermal fibroblasts embedded in a 3D collagen lattice
Peters AS, Brunner G, Krieg T, Eckes B. Arch Dermatol Res 2014 Oct 28.
Effects of physiologic mechanical stimulation on embryonic chick cardiomyocytes using a microfluidic cardiac cell culture model
Nguyen MD, Tinney JP, Ye F, Elnakib AA, Yuan F, El-Baz A, Sethu P, Keller BB, Giridharan GA. Anal Chem87(4):2107-13, 2015. doi: 10.1021/ac503716z. Epub 2015 Feb 2.
Cyclic tensile strain upon human mesenchymal stem cells in 2D and 3D culture differentially influences CCNL2, WDR61 and BAHCC1 gene expression levels
Rathbone SR, Glossop JR, Gough JE, Cartmell SH. J Mech Behav Biomed Mater 11:82-91, 2012.
Mechanical stress promotes maturation of human myocardium from pluripotent stem cell-derived progenitors
Ruan JL, Tulloch NL, Saiget M, Paige SL, Razumova MV, Regnier M, Tung KC, Keller G, Pabon L, Reinecke H, Murry CE. Stem Cells 33(7):2148-57, 2015. doi: 10.1002/stem.2036. Epub 2015 May 11.
Effects of intermittent and incremental cyclic stretch on ERK signaling and collagen production in engineered tissue
Schmidt JB, Chen K, Tranquillo RT. Cellular and Molecular Bioengineering 1-10, 2015. doi:10.1007/s12195-015-0415-6.
Combined biophysical and soluble factor modulation induces cardiomyocyte differentiation from human muscle derived stem cells
Tchao J, Han L, Lin B, Yang L, Tobita K. Sci Rep 4:6614, 2014. doi: 10.1038/srep06614.
Mechanical stretch assays in cell culture systems
Tondon A, Haase C, Kaunas R. In: Handbook of Imaging in Biological Mechanics, ed. Neu CP, Genin GM. CRC Press: Boca Raton, 2015.
Engineered human muscle tissue from skeletal muscle derived stem cells and induced pluripotent stem cell derived cardiac cells
Tchao J, Kim JJ, Lin B, Salama G, Lo CW, Yang L, Tobita K. International Journal of Tissue Engineering 2013Article ID 198762, 15 pages, 2013. http://dx.doi.org/10.1155/2013/198762.
Combating adaptation to cyclic stretching by prolonging activation of extracellular signal-regulated kinase
Weinbaum JS, Schmidt JB, Tranquillo RT. Cellular and Molecular Bioengineering 6 (3):279-286, 2013.
Enhancement of tenogenic differentiation of human adipose stem cells by tendon-derived extracellular matrix
Yang G, Rothrauff BB, Lin H, Gottardi R, Alexander PG, Tuan RS. Biomaterials 34(37):9295-306, 2013. doi: 10.1016/j.biomaterials.2013.08.054.
Application of polarization-sensitive OCT and Doppler OCT in tissue engineering
Yang Y, Wimpenny I, Wang RK. In: Optical Techniques in Regnerative Medicine, edited by Morgan SP, Rose F, Matcher SJ. Taylor & Francis Group: Florida, p. 307-327, 2014.
Gene expression profiles in engineered cardiac tissues respond to mechanical loading and inhibition of tyrosine kinases
Ye F, Yuan F, Li X, Cooper N, Tinney JP, Keller BB. Physiol Rep 1(5):e00078, 2013. doi: 10.1002/phy2.78.
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